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Selectable types of Cell mirror fluorescence filters
As far as the fluorescent cell mirror is concerned, the excitation filter, dichroic mirror, and emission filter are usually contained in the same cube and are usually designed to match the spectra of specific fluorophores or fluorescent proteins. Occasionally, the filter may not be inside the cube, but will be installed on a wheel (called a filter wheel).
Cell mirror fluorescence filter What types of filters are used for fluorescence imaging?
Under normal circumstances, the product is designed to capture the excitation and emission wavelengths of the specified fluorophore, but not all the fluorescence. Most modern designs are designed to ensure that the excitation product allows light in the specified wavelength range to pass.
This type is called a bandpass filter. It is usually determined by the intermediate wavelength and the interval width. In the simpler fluorescent Cell mirror device, once the excitation light leaves the excitation filter, it will illuminate the target, and the fluorophore on the target will immediately become an excited state, and then emit to the infrared end of the spectrum (relative to the excitation light ) Migrating light, not all such emitted light will be captured by the detector-only light that is allowed to pass through the dichroic mirror and can pass through the emission filter.
Cell mirror emission filters are usually band-pass filters or long-wave-pass filters, depending on the specific fluorophore and imaging experiment. If you want light exceeding a certain wavelength to pass through the detector, you can choose a long-pass filter.